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Briefly, FreSHtracer was synthesized by mixing a coumarin-3-carbaldehyde derivative and 2-cyano- N,N -dimethylacetamide at 60 C for 2 days, and purified using SiO 2 chromatography [19]

with a final elongation at 72 C for 10 min

Senescent ECs accumulate in arterial tissues throughout the human lifespan and are positively associated with atherosclerosis [9, 10]

Preservation of the highest (millimolar) concentrations of reduced GSH in most cell types highlights GSH vital and multifunctional roles in controlling various biological processes like detoxification of foreign and endogenous compounds, protein folding, regeneration of vitamins C and E, maintenance of mitochondrial function, regulation of cell cycle and cell proliferation, apoptosis, immune response, and multiple other cellular and biological functions, particularly important, antiviral defense (Meister and Anderson, 1983

doi: 10.1002/ps.3710 206 YuJ.WuS.SunH.WangX.TangX.GuoS.et al